SMAD6
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- cell-substrate adhesion [IMP]
- fat cell differentiation [IDA]
- immune response [IMP]
- intracellular signal transduction [IDA, IMP]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of SMAD protein complex assembly [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to laminar fluid shear stress [IEP]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
NEDD4
Gene Ontology Biological Process
- cellular response to UV [IMP]
- cytokine-mediated signaling pathway [TAS]
- development involved in symbiotic interaction [IMP]
- glucocorticoid receptor signaling pathway [IDA]
- lysosomal transport [IDA]
- negative regulation of sodium ion transport [IDA]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [IMP]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- neuron projection development [IEP]
- positive regulation of nucleocytoplasmic transport [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of protein catabolic process [IDA]
- progesterone receptor signaling pathway [IDA]
- protein K63-linked ubiquitination [ISS]
- protein targeting to lysosome [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- receptor catabolic process [IDA]
- receptor internalization [IDA]
- regulation of dendrite morphogenesis [ISS]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane potential [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- response to calcium ion [TAS]
- transmission of virus [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor.
Inhibitory Smad, Smad7, is a potent inhibitor of TGF-beta (transforming growth factor-beta) superfamily signalling. By binding to activated type I receptors, it prevents the activation of R-Smads (receptor-regulated Smads). To identify new components of the Smad pathway, we performed yeast two-hybrid screening using Smad7 as bait, and identified NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) as a direct binding ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID