NANOG
Gene Ontology Biological Process
- cell proliferation [ISO]
- embryo development [IMP]
- endodermal cell fate specification [ISO]
- mesodermal cell fate commitment [IGI]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell differentiation [IDA]
- negative regulation of cell fate commitment [IDA, IGI]
- negative regulation of endodermal cell fate specification [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- positive regulation of cell proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of cell differentiation [ISO]
- regulation of gene expression [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA, IMP]
- stem cell division [IDA]
- stem cell maintenance [IDA, IMP]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA, ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IMP, ISO]
- transcription corepressor activity [IMP]
- transcription regulatory region DNA binding [IDA]
MTA2
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [ISO]
- DNA methylation [IMP]
- DNA packaging [TAS]
- chromatin remodeling [TAS]
- histone deacetylation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of fibroblast migration [ISO]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells.
Nanog and Oct4 are essential transcription factors that regulate self-renewal and pluripotency of ES cells. However, the mechanisms by which Nanog and Oct4 modulate ES cell fate remain unknown. Through characterization of endogenous Nanog and Oct4 protein complexes in mouse ES cells, we found that these transcription factors interact with each other and associate with proteins from multiple repression complexes, ... [more]
Throughput
- Low Throughput
Additional Notes
- escells
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NANOG MTA2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| NANOG MTA2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 924 | BioGRID | 885903 | |
| NANOG MTA2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 677394 | |
| NANOG MTA2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 882748 | |
| NANOG MTA2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 471879 | |
| MTA2 NANOG | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID