BAIT
HPR1
TRF1, L000000808, YDR138W
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
ELG1
RTT110, S000007438, YOR144C
Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
GO Process (7)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.
Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination, the mechanisms by which they trigger recombination are still elusive. In order to understand how R-loops compromise genome stability, we have analyzed the genetic interaction of THO with 26 genes involved ... [more]
Mol. Cell. Biol. Oct. 01, 2009; 29(19);5203-13 [Pubmed: 19651896]
Throughput
- Low Throughput
Ontology Terms
- phenotype: chromosome/plasmid maintenance (APO:0000143)
- phenotype: inviable (APO:0000112)
- phenotype: resistance to chemicals (APO:0000087)
Additional Notes
- double mutants show decreased chromosome stability through increased RAD52 foci, GCR or recombination
- double mutants show increased sensitivity to HU and MMS
Curated By
- BioGRID