BAIT

HPR1

TRF1, L000000808, YDR138W
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
Saccharomyces cerevisiae (S288c)
PREY

PRI1

DNA primase subunit PRI1, L000001489, YIR008C
Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
GO Process (4)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.

Gomez-Gonzalez B, Felipe-Abrio I, Aguilera A

Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination, the mechanisms by which they trigger recombination are still elusive. In order to understand how R-loops compromise genome stability, we have analyzed the genetic interaction of THO with 26 genes involved ... [more]

Mol. Cell. Biol. Oct. 01, 2009; 29(19);5203-13 [Pubmed: 19651896]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • double mutants show increased sensitivity to HU and MMS

Curated By

  • BioGRID