BAIT

HPR1

TRF1, L000000808, YDR138W
Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
Saccharomyces cerevisiae (S288c)
PREY

RAD24

RS1, L000001777, YER173W
Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants.

Gomez-Gonzalez B, Felipe-Abrio I, Aguilera A

Cotranscriptional R-loops are formed in yeast mutants of the THO complex, which functions at the interface between transcription and mRNA export. Despite the relevance of R-loops in transcription-associated recombination, the mechanisms by which they trigger recombination are still elusive. In order to understand how R-loops compromise genome stability, we have analyzed the genetic interaction of THO with 26 genes involved ... [more]

Mol. Cell. Biol. Oct. 01, 2009; 29(19);5203-13 [Pubmed: 19651896]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome/plasmid maintenance (APO:0000143)
  • phenotype: vegetative growth (APO:0000106)
  • phenotype: spore germination (APO:0000042)

Additional Notes

  • double mutants show decreased chromosome stability through increased RAD52 foci, GCR or recombination

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HPR1 RAD24
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
3458960
HPR1 RAD24
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
478012

Curated By

  • BioGRID