A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Critical functions of rpa3/ssb3 in s-phase DNA damage responses in fission yeast.

Cavero S, Limbo O, Russell P

Replication Protein A (RPA) is a heterotrimeric, single-stranded DNA (ssDNA)-binding complex required for DNA replication and repair, homologous recombination, DNA damage checkpoint signaling, and telomere maintenance. Whilst the larger RPA subunits, Rpa1 and Rpa2, have essential interactions with ssDNA, the molecular functions of the smallest subunit Rpa3 are unknown. Here, we investigate the Rpa3 ortholog Ssb3 in Schizosaccharomyces pombe and ... [more]

PLoS Genet. Oct. 05, 2010; 6(9); [Pubmed: 20885790]

Throughput

Low Throughput

Ontology Terms

phenotype: uv resistance (APO:0000085)
phenotype: vegetative growth (APO:0000106)
phenotype: resistance to chemicals (APO:0000087)

Additional Notes

double mutants show increased sensitivity to CPT, MMS, HU and UV radiation

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRC1 SSB3	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-3.5034	BioGRID	756785

Curated By

BioGRID

Interaction Summary

SSB3

Gene Ontology Biological Process

Gene Ontology Molecular Function

single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

BRC1