An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.

Ueda J, Tachibana M, Ikura T, Shinkai Y

G9a is a SET-domain mammalian histone methyltransferase responsible for mono- and dimethylation of lysine 9 in histone H3 (H3K9) at euchromatic regions. Recently we reported that G9a forms a stoichiometric heteromeric complex with another SET-domain-containing molecule, GLP/Eu-HMTase1. Although G9a and GLP can independently methylate H3K9 in vitro, G9a/GLP heteromeric formation seems to be essential for their function as a euchromatic ... [more]

J. Biol. Chem. Jul. 21, 2006; 281(29);20120-8 [Pubmed: 16702210]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

EHMT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

C2H2 zinc finger domain binding [IPI]histone methyltransferase activity (H3-K27 specific) [ISS]histone methyltransferase activity (H3-K9 specific) [ISS]histone-lysine N-methyltransferase activity [IMP]p53 binding [IPI]protein binding [IPI]protein-lysine N-methyltransferase activity [IDA]

Gene Ontology Cellular Component

EHMT1