An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains.

Liu PY, Hsieh TY, Chou WY, Huang SM

Glucocorticoid receptor-interacting protein 1 (GRIP1), a p160 family nuclear receptor co-activator, possesses at least two autonomous activation domains (AD1 and AD2) in the C-terminal region. AD1 activity appears to be mediated by CBP/p300, whereas AD2 activity is apparently mediated through co-activator-associated arginine methyltransferase 1 (CARM1). The mechanisms responsible for regulating the activities of AD1 and AD2 are not well understood. ... [more]

FEBS J. May. 01, 2006; 273(10);2172-83 [Pubmed: 16649994]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRIP1 GRIP1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Wu M (2004)	Low	-	BioGRID	-
GRIP1 GRIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Liu PY (2006)	Low	-	BioGRID	-
GRIP1 GRIP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wu M (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GRIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [NAS]beta-catenin binding [TAS]glucocorticoid receptor binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]receptor signaling complex scaffold activity [NAS]transcription coactivator activity [NAS]

Gene Ontology Cellular Component