BAIT

SPT21

L000002040, YMR179W
Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

HHT2

histone H3, L000000773, YNL031C
Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Spt10 and Spt21 are required for transcriptional silencing in Saccharomyces cerevisiae.

Chang JS, Winston F

In Saccharomyces cerevisiae, transcriptional silencing occurs at three classes of genomic regions: near the telomeres, at the silent mating type loci, and within the rDNA repeats. In all three cases, silencing depends upon several factors, including specific types of histone modifications. In this work we have investigated the roles in silencing for Spt10 and Spt21, two proteins previously shown to ... [more]

Unknown Nov. 05, 2010; 0(0); [Pubmed: 21057056]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: mating efficiency (APO:0000034)
  • phenotype: silencing (APO:0000046)

Additional Notes

  • genetic complex
  • overexpression of the histone genes can strongly suppress defects in silencing caused by deletion of spt21

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HHT2 SPT21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.4038BioGRID
217101
SPT21 HHT2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-7.3884BioGRID
510308
SPT21 HHT2
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
2901287

Curated By

  • BioGRID