An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.

Takai H, Xie Y, de Lange T, Pavletich NP

We reported previously that the stability of all mammalian phosphatidylinositol 3-kinase-related protein kinases (PIKKs) depends on their interaction with Tel2, the ortholog of yeast Tel2 and Caenorhabditis elegans Clk-2. Here we provide evidence that Tel2 acts with Hsp90 in the maturation of PIKK complexes. Quantitative immunoblotting showed that the abundance of Tel2 is low compared with the PIKKs, and Tel2 ... [more]

Genes Dev. Sep. 15, 2010; 24(18);2019-30 [Pubmed: 20801936]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

TELO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

DNAJB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activator activity [IDA]ATPase binding [IPI]Hsp70 protein binding [IPI]chaperone binding [IPI]protein binding [IPI]unfolded protein binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.

Throughput

Curated By

protein binding [IPI]
protein complex binding [IDA]

ATPase activator activity [IDA]
ATPase binding [IPI]
Hsp70 protein binding [IPI]
chaperone binding [IPI]
protein binding [IPI]
unfolded protein binding [IDA]