SMAD6
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- cell-substrate adhesion [IMP]
- fat cell differentiation [IDA]
- immune response [IMP]
- intracellular signal transduction [IDA, IMP]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of SMAD protein complex assembly [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to laminar fluid shear stress [IEP]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
SMURF2
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- gene expression [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [NAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]
- regulation of transforming growth factor beta receptor signaling pathway [NAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IBA, TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Negative regulation of transforming growth factor-beta (TGF-beta) signaling by WW domain-containing protein 1 (WWP1).
Smad7 negatively regulates transforming growth factor (TGF)-beta superfamily signaling by binding to activated type I receptors, thereby preventing the phosphorylation of receptor-regulated Smads (R-Smads), as well as by recruiting HECT-type E3 ubiquitin ligases to degrade type I receptors through a ubiquitin-dependent mechanism. To elucidate the regulatory mechanisms of TGF-beta signaling, we searched for novel members of proteins that interact with ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD6 SMURF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| SMURF2 SMAD6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID