H2AFX
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PABPC1
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- mRNA metabolic process [TAS]
- mRNA polyadenylation [TAS]
- mRNA splicing, via spliceosome [IC]
- mRNA stabilization [TAS]
- negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- positive regulation of translation [TAS]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The dynamic alterations of H2AX complex during DNA repair detected by a proteomic approach reveal the critical roles of Ca(2+)/calmodulin in the ionizing radiation-induced cell cycle arrest.
By using DNA nuclease digestion and a quantitative "dual tagging" proteomic approach that integrated mass spectrometry, stable isotope labeling, and affinity purification, we studied the histone H2AX-associating protein complex in chromatin in mammalian cells in response to ionizing radiation (IR). In the non-irradiated control cells, calmodulin (CaM) and the transcription elongation factor facilitates chromatin transcription (FACT) were associated with H2AX. ... [more]
Throughput
- Low Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| H2AFX PABPC1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 558169 | |
| PABPC1 H2AFX | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3437474 |
Curated By
- BioGRID