Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The dynamic alterations of H2AX complex during DNA repair detected by a proteomic approach reveal the critical roles of Ca(2+)/calmodulin in the ionizing radiation-induced cell cycle arrest.

Du YC, Gu S, Zhou J, Wang T, Cai H, Macinnes MA, Bradbury EM, Chen X

By using DNA nuclease digestion and a quantitative "dual tagging" proteomic approach that integrated mass spectrometry, stable isotope labeling, and affinity purification, we studied the histone H2AX-associating protein complex in chromatin in mammalian cells in response to ionizing radiation (IR). In the non-irradiated control cells, calmodulin (CaM) and the transcription elongation factor facilitates chromatin transcription (FACT) were associated with H2AX. ... [more]

Mol. Cell Proteomics Jun. 01, 2006; 5(6);1033-44 [Pubmed: 16522924]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181)

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID