BAIT

RAD9

chromatin-binding protein RAD9, L000001562, YDR217C
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
Saccharomyces cerevisiae (S288c)
PREY

POL30

proliferating cell nuclear antigen, PCNA, L000001463, YBR088C
Proliferating cell nuclear antigen (PCNA); functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The Role of Replication Bypass Pathways in Dicentric Chromosome Formation in Budding Yeast.

Paek AL, Jones H, Kaochar S, Weinert T

Gross chromosomal rearrangements (GCRs) are large scale changes to chromosome structure, and can lead to human disease. We previously showed in Saccharomyces cerevisiae, that nearby inverted repeat sequences (~20 to 200bp of homology, separated by ~1-5kb) frequently fuse to form unstable dicentric and acentric chromosomes. Here we analyzed inverted repeat fusion in mutants of three sets of genes. First, we ... [more]

Unknown Sep. 13, 2010; 0(0); [Pubmed: 20837992]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome segregation (APO:0000208)
  • phenotype: mutation frequency (APO:0000198)

Additional Notes

  • double mutants show increased mixed colony formation indicative of increased mutation during DNA replication

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD9 POL30
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-11.7377BioGRID
213789
POL30 RAD9
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
162934

Curated By

  • BioGRID