BAIT

RAD9

chromatin-binding protein RAD9, L000001562, YDR217C
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
Saccharomyces cerevisiae (S288c)
PREY

MGS1

ssDNA-dependent ATPase MGS1, YNL218W
Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
UPS Project
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The Role of Replication Bypass Pathways in Dicentric Chromosome Formation in Budding Yeast.

Paek AL, Jones H, Kaochar S, Weinert T

Gross chromosomal rearrangements (GCRs) are large scale changes to chromosome structure, and can lead to human disease. We previously showed in Saccharomyces cerevisiae, that nearby inverted repeat sequences (~20 to 200bp of homology, separated by ~1-5kb) frequently fuse to form unstable dicentric and acentric chromosomes. Here we analyzed inverted repeat fusion in mutants of three sets of genes. First, we ... [more]

Unknown Sep. 13, 2010; 0(0); [Pubmed: 20837992]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome segregation (APO:0000208)
  • phenotype: mutation frequency (APO:0000198)

Additional Notes

  • double mutants show decreased mixed colony formation indicative of decreased mutation during DNA replication

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD9 MGS1
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Low-BioGRID
253634

Curated By

  • BioGRID