BAIT

HST4

L000003043, YDR191W
Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RFC1

CDC44, replication factor C subunit 1, L000000278, YOR217W
Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
GO Process (4)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Histone H3 K56 hyperacetylation perturbs replisomes and causes DNA damage.

Celic I, Verreault A, Boeke JD

Deacetylation of histone H3 K56, regulated by the sirtuins Hst3p and Hst4p, is critical for maintenance of genomic stability. However, the physiological consequences of a lack of H3 K56 deacetylation are poorly understood. Here we show that cells lacking Hst3p and Hst4p, in which H3 K56 is constitutively hyperacetylated, exhibit hallmarks of spontaneous DNA damage, such as activation of the ... [more]

Genetics Aug. 01, 2008; 179(4);1769-84 [Pubmed: 18579506]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: temperature sensitive growth (APO:0000092)
  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: uv resistance (APO:0000085)

Additional Notes

  • Overexpression of RFC1 rescues growth of hst3/hst4 mutants under various stress conditions
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RFC1 HST4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1246BioGRID
2017727

Curated By

  • BioGRID