An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Histone deacetylase-associating Atrophin proteins are nuclear receptor corepressors.

Wang L, Rajan H, Pitman JL, McKeown M, Tsai CC

Drosophila Tailless (Tll) is an orphan nuclear receptor involved in embryonic segmentation and neurogenesis. Although Tll exerts potent transcriptional repressive effects, the underlying molecular mechanisms have not been determined. Using the established regulation of knirps by tll as a paradigm, we report that repression of knirps by Tll involves Atrophin, which is related to vertebrate Atrophin-1 and Atrophin-2. Atrophin interacts ... [more]

Genes Dev. Mar. 01, 2006; 20(5);525-30 [Pubmed: 16481466]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NR2E1 ATN1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Estruch SB (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein domain specific binding [IPI]transcription corepressor activity [IDA]

Gene Ontology Cellular Component

NR2E1