BAIT

MP

ARF5, AUXIN RESPONSE FACTOR 5, F6F9.10, F6F9_10, IAA24, MONOPTEROS, TRANSCRIPTION FACTOR IAA24, indole-3-acetic acid inducible 24, AT1G19850
auxin response factor 5
Arabidopsis thaliana (Columbia)
PREY

BRX

BREVIS RADIX, F5M6.11, F5M6_11, NIP3;1, NLM9, NOD26-like intrinsic protein 3;1, AT1G31880
protein BREVIS RADIX
GO Process (5)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Spatio-temporal sequence of cross-regulatory events in root meristem growth.

Scacchi E, Salinas P, Gujas B, Santuari L, Krogan N, Ragni L, Berleth T, Hardtke CS

A central question in developmental biology is how multicellular organisms coordinate cell division and differentiation to determine organ size. In Arabidopsis roots, this balance is controlled by cytokinin-induced expression of SHORT HYPOCOTYL 2 (SHY2) in the so-called transition zone of the meristem, where SHY2 negatively regulates auxin response factors (ARFs) by protein-protein interaction. The resulting down-regulation of PIN-FORMED (PIN) auxin ... [more]

Unknown Dec. 13, 2010; 0(0); [Pubmed: 21149702]

Throughput

  • Low Throughput

Curated By

  • BioGRID