BAIT
PIF1
TST1, DNA helicase PIF1, L000001435, YML061C
DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; important for crossover recombination; translation from different start sites produces mitochondrial and nuclear forms; nuclear form is a catalytic inhibitor of telomerase; mitochondrial form involved in DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
GO Process (9)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- DNA recombination [IMP]
- DNA strand renaturation [IDA]
- G-quadruplex DNA unwinding [IMP]
- chromosome organization [IMP]
- double-strand break repair via break-induced replication [IMP]
- mitochondrial genome maintenance [IMP]
- negative regulation of telomere maintenance via telomerase [IDA, IMP]
- telomere maintenance [IMP]
- telomere maintenance via recombination [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
EXO1
DHS1, Rad2 family nuclease EXO1, L000000505, L000003929, YOR033C
5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
GO Process (6)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping.
Essential telomere 'capping' proteins act as a safeguard against ageing and cancer by inhibiting the DNA damage response (DDR) and regulating telomerase recruitment, thus distinguishing telomeres from double-strand breaks (DSBs). Uncapped telomeres and unrepaired DSBs can both stimulate a potent DDR, leading to cell cycle arrest and cell death. Using the cdc13-1 mutation to conditionally 'uncap' telomeres in budding yeast, ... [more]
EMBO J. Dec. 01, 2010; 29(23);4020-34 [Pubmed: 21045806]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- cdc13/pif1/exo1/rad52 quadruple mutants are lethal
- cdc13/pif1/exo1/tlc1 quadruple mutants are lethal
- cdc13/pif1/exo1/yku70 quadruple mutants are lethal
- genetic complex
Curated By
- BioGRID