BAIT

GHR

GHBP
growth hormone receptor
GO Process (27)
GO Function (6)
GO Component (8)
Homo sapiens
PREY

PTPN1

PTP1B
protein tyrosine phosphatase, non-receptor type 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of protein tyrosine phosphatases with specificity for the ligand-activated growth hormone receptor.

Pasquali C, Curchod ML, Waelchli S, Espanel X, Guerrier M, Arigoni F, Strous G, Hooft van Huijsduijnen R

Protein tyrosine phosphatases (PTPs) play key roles in switching off tyrosine phosphorylation cascades, such as initiated by cytokine receptors. We have used substrate-trapping mutants of a large set of PTPs to identify members of the PTP family that have substrate specificity for the phosphorylated human GH receptor (GHR) intracellular domain. Among 31 PTPs tested, T cell (TC)-PTP, PTP-beta, PTP1B, stomach ... [more]

Mol. Endocrinol. Nov. 01, 2003; 17(11);2228-39 [Pubmed: 12907755]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GHR PTPN1
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
-

Curated By

  • BioGRID