BAIT
MGMT
RP11-109A6.1
O-6-methylguanine-DNA methyltransferase
GO Process (4)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
KPNB1
IMB1, IPO1, IPOB, Impnb, NTF97
karyopherin (importin) beta 1
GO Process (12)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [TAS]
- apoptotic DNA fragmentation [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cytokine-mediated signaling pathway [TAS]
- intracellular transport of virus [TAS]
- protein import into nucleus [IDA]
- protein import into nucleus, translocation [TAS]
- ribosomal protein import into nucleus [IDA]
- small molecule metabolic process [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of human O6-methylguanine-DNA methyltransferase by affinity chromatography and tandem mass spectrometry.
Recent evidence suggests that human O(6)-methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that protects the genome against mutagens and accords tumor resistance to many anticancer alkylating agents, may have other roles besides repair. Therefore, we isolated MGMT-interacting proteins from extracts of HT29 human colon cancer cells using affinity chromatography on MGMT-Sepharose. Specific proteins bound to this column were identified by ... [more]
Biochem. Biophys. Res. Commun. Dec. 02, 2005; 337(4);1176-84 [Pubmed: 16226712]
Throughput
- Low Throughput
Curated By
- BioGRID