BAIT
MGMT
RP11-109A6.1
O-6-methylguanine-DNA methyltransferase
GO Process (4)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
EEF1B2
EEF1B, EEF1B1, EF1B
eukaryotic translation elongation factor 1 beta 2
GO Process (4)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of human O6-methylguanine-DNA methyltransferase by affinity chromatography and tandem mass spectrometry.
Recent evidence suggests that human O(6)-methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that protects the genome against mutagens and accords tumor resistance to many anticancer alkylating agents, may have other roles besides repair. Therefore, we isolated MGMT-interacting proteins from extracts of HT29 human colon cancer cells using affinity chromatography on MGMT-Sepharose. Specific proteins bound to this column were identified by ... [more]
Biochem. Biophys. Res. Commun. Dec. 02, 2005; 337(4);1176-84 [Pubmed: 16226712]
Throughput
- Low Throughput
Curated By
- BioGRID