An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and Characterization of Nuclear Pore Complex Components in Arabidopsis thaliana.

Tamura K, Fukao Y, Iwamoto M, Haraguchi T, Hara-Nishimura I

The nuclear pore complex (NPC) facilitates nucleocytoplasmic transport, a crucial process for various cellular activities. The NPC comprises ~30 nucleoporins and is well characterized in vertebrates and yeast. However, only eight plant nucleoporins have been identified, and little information is available about the complete molecular structure of plant NPCs. In this study, an interactive proteomic approach was used to identify ... [more]

Unknown Dec. 28, 2010; 0(0); [Pubmed: 21189294]

Throughput

High Throughput|Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT1G52380 IMPA-2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Braun P (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT1G52380

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

IMPA-2

Gene Ontology Biological Process