BAIT

PHO90

YJL198W
Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PHO4

phosphate-sensing transcription factor PHO4, phoD, L000001420, YFR034C
Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae.

Ghillebert R, Swinnen E, De Snijder P, Smets B, Winderickx J

When starved for phosphate (Pi), yeast cells activate the PHO-signaling pathway, wherein the Pho4 transcription factor mediates expression of genes involved in Pi-acquisition, such as PHO84, encoding the high-affinity H+/Pi-symporter. In contrast, transcription of PHO87 and PHO90, encoding the low-affinity H+/Pi-transport system, is independent of phosphate status. Here, we reveal that upon Pi-starvation, these low-affinity phosphate transporters are endocytosed and ... [more]

Unknown Dec. 10, 2010; 0(0); [Pubmed: 21143198]

Throughput

  • Low Throughput

Ontology Terms

  • utilization of phosphorus source (APO:0000125)
  • vegetative growth (APO:0000106)

Additional Notes

  • double mutants show a growth defect at in low phosphate media

Curated By

  • BioGRID