BAIT

PHO90

YJL198W
Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PHO89

ITN1, YBR296C
Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae.

Ghillebert R, Swinnen E, De Snijder P, Smets B, Winderickx J

When starved for phosphate (Pi), yeast cells activate the PHO-signaling pathway, wherein the Pho4 transcription factor mediates expression of genes involved in Pi-acquisition, such as PHO84, encoding the high-affinity H+/Pi-symporter. In contrast, transcription of PHO87 and PHO90, encoding the low-affinity H+/Pi-transport system, is independent of phosphate status. Here, we reveal that upon Pi-starvation, these low-affinity phosphate transporters are endocytosed and ... [more]

Unknown Dec. 10, 2010; 0(0); [Pubmed: 21143198]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: utilization of phosphorus source (APO:0000125)

Additional Notes

  • genetic complex
  • triple mutants show a growth defect at in low phosphate media

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHO89 PHO90
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
452662

Curated By

  • BioGRID