AURKB
Gene Ontology Biological Process
- cellular response to UV [ISO]
- cleavage furrow formation [ISO]
- histone H3-S28 phosphorylation [IDA]
- mitotic spindle midzone assembly [ISO]
- mitotic spindle organization [IBA]
- negative regulation of B cell apoptotic process [ISO]
- negative regulation of protein binding [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of cytokinesis [ISO]
- protein localization to kinetochore [ISO]
- protein phosphorylation [IBA, ISO]
- regulation of cytokinesis [IBA]
- spindle stabilization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- chromocenter [IDA]
- chromosome passenger complex [IDA, ISO]
- condensed chromosome, centromeric region [ISO]
- condensed nuclear chromosome, centromeric region [IBA, ISO]
- kinetochore [ISO]
- midbody [IDA, ISO]
- mitotic spindle pole [ISO]
- nucleus [ISO]
- spindle [ISO]
- spindle microtubule [IBA]
- spindle midzone [IBA]
- spindle pole centrosome [IBA]
PARP1
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [ISO]
- DNA metabolic process [IMP]
- DNA repair [TAS]
- base-excision repair [IMP]
- cellular response to insulin stimulus [ISO]
- cellular response to superoxide [IDA]
- double-strand break repair [IGI, ISO]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein ADP-ribosylation [IBA, ISO]
- protein autoprocessing [ISO]
- protein poly-ADP-ribosylation [ISO]
- regulation of growth rate [IMP]
- signal transduction involved in regulation of gene expression [ISO]
- telomere maintenance [IMP]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Inhibition of Aurora-B kinase activity by poly(ADP-ribosyl)ation in response to DNA damage.
The cell cycle-regulated Aurora-B kinase is a chromosomal passenger protein that is implicated in fundamental mitotic events, including chromosome alignment and segregation and spindle checkpoint function. Aurora-B phosphorylates serine 10 of histone H3, a function that has been associated with mitotic chromatin condensation. We find that activation of poly(ADP-ribose) polymerase (PARP) 1 by DNA damage results in a rapid block ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AURKB PARP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PARP1 AURKB | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PARP1 AURKB | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 482672 | |
| PARP1 AURKB | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - |
Curated By
- BioGRID