BAIT

APC5

RMC1, anaphase promoting complex subunit 5, L000004320, YOR249C
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
UPS Project
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

DOC1

APC10, anaphase promoting complex subunit DOC1, L000004350, YGL240W
Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain
UPS Project
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The yeast Anaphase Promoting Complex interacts with multiple histone modifying enzymes to regulate cell cycle progression.

Turner EL, Malo ME, Pisclevich MG, Dash MD, Davies GF, Arnason TG, Harkness TA

The Anaphase Promoting Complex (APC), a large evolutionarily conserved ubiquitin-ligase complex, regulates cell cycle progression through mitosis and G1. Here, we present data suggesting APC-dependent cell cycle progression relies on a specific set of histone post-translational modifying enzymes. Multiple APC subunit mutants were impaired in total and modified histone H3 protein content. H3K56(Ac) levels were as reduced as total H3, ... [more]

Unknown Aug. 13, 2010; 0(0); [Pubmed: 20709786]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide modification (APO:0000131)
  • phenotype: resistance to chemicals (APO:0000087)
  • phenotype: uv resistance (APO:0000085)

Additional Notes

  • A temperature-sensitive apc5-CA allele was used in the experiment.
  • Methyl methanesulfonate (MMS) (CHEBI:25255 and PubChem CID: 4156)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DOC1 APC5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
DOC1 APC5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High9BioGRID
3619721
APC5 DOC1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
APC5 DOC1
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
435180
APC5 DOC1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
157597

Curated By

  • BioGRID