VMA21
Gene Ontology Biological Process
Gene Ontology Cellular Component
VPS29
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
A Genome-wide Enhancer Screen Implicates Sphingolipid Composition in Vacuolar ATPase Function in Saccharomyces cerevisiae.
The function of the vacuolar H(+)-ATPase enzyme complex is to acidify organelles; this process is critical for a variety of cellular processes and has implications in human disease. There are five accessory proteins that assist in assembly of the membrane portion of the complex, the V(0) domain. To identify additional elements that affect V-ATPase assembly, trafficking, or enzyme activity, we ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: metal resistance (APO:0000090)
Additional Notes
- A synthetic genetic array (SGA) screen was used to identify genes that caused an increase in calcium or zinc sensitivity in a VMA21 mutant with a dysfunctional ER retrieval motif (vma21QQ).
- A third SGA screen was also used to identify genes that caused an increase in calcium or zinc sensitivity in a vma21QQ voa1::Nat-R double mutant. Complete deletion of VOA1 (voa1::Nat-R) results in a decrease in steady-state levels of Vma21p.
- Another SGA screen was also used to identify genes that caused an increase in calcium or zinc sensitivity in a vma21QQ voa1::Hyg-R double mutant. VOA1 is genetically linked to the VMA21 locus. The voa1::Hyg-R allele enhances the V-ATPase deficiency of a vma21QQ mutant which has a dysfunctional ER retrieval motif.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VMA21 VPS29 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1241 | BioGRID | 382465 | |
| VPS29 VMA21 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1241 | BioGRID | 384920 | |
| VMA21 VPS29 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.185 | BioGRID | 2121101 |
Curated By
- BioGRID