BAIT

VMA21

L000002466, YGR105W
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

AAT2

ASP5, aspartate transaminase AAT2, L000000131, L000002677, YLR027C
Cytosolic aspartate aminotransferase involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

A Genome-wide Enhancer Screen Implicates Sphingolipid Composition in Vacuolar ATPase Function in Saccharomyces cerevisiae.

Finnigan GC, Ryan M, Stevens TH

The function of the vacuolar H(+)-ATPase enzyme complex is to acidify organelles; this process is critical for a variety of cellular processes and has implications in human disease. There are five accessory proteins that assist in assembly of the membrane portion of the complex, the V(0) domain. To identify additional elements that affect V-ATPase assembly, trafficking, or enzyme activity, we ... [more]

Unknown Dec. 31, 2010; 0(0); [Pubmed: 21196517]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: metal resistance (APO:0000090)

Additional Notes

  • A synthetic genetic array (SGA) screen was used to identify genes that caused an increase in calcium or zinc sensitivity in a VMA21 mutant with a dysfunctional ER retrieval motif (vma21QQ).
  • Another SGA screen was also used to identify genes that caused an increase in calcium or zinc sensitivity in a vma21QQ voa1::Nat-R double mutant. Complete deletion of VOA1 (voa1::Nat-R) results in a decrease in steady-state levels of Vma21p.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AAT2 VMA21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1481BioGRID
396927

Curated By

  • BioGRID