BAIT

VMA21

L000002466, YGR105W
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ACO2

aconitate hydratase ACO2, YJL200C
Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

A Genome-wide Enhancer Screen Implicates Sphingolipid Composition in Vacuolar ATPase Function in Saccharomyces cerevisiae.

Finnigan GC, Ryan M, Stevens TH

The function of the vacuolar H(+)-ATPase enzyme complex is to acidify organelles; this process is critical for a variety of cellular processes and has implications in human disease. There are five accessory proteins that assist in assembly of the membrane portion of the complex, the V(0) domain. To identify additional elements that affect V-ATPase assembly, trafficking, or enzyme activity, we ... [more]

Unknown Dec. 31, 2010; 0(0); [Pubmed: 21196517]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: metal resistance (APO:0000090)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • A synthetic genetic array (SGA) screen was used to identify genes that caused an increase in calcium or zinc sensitivity in a VMA21 mutant with a dysfunctional ER retrieval motif (vma21QQ).

Curated By

  • BioGRID