BAIT

VMA21

L000002466, YGR105W
Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human XMEA (X-linked Myopathy with Excessive Autophagy); functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SRN2

SRN10, VPL16, VPS37, ESCRT-I subunit protein SRN2, L000003103, YLR119W
Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

A Genome-wide Enhancer Screen Implicates Sphingolipid Composition in Vacuolar ATPase Function in Saccharomyces cerevisiae.

Finnigan GC, Ryan M, Stevens TH

The function of the vacuolar H(+)-ATPase enzyme complex is to acidify organelles; this process is critical for a variety of cellular processes and has implications in human disease. There are five accessory proteins that assist in assembly of the membrane portion of the complex, the V(0) domain. To identify additional elements that affect V-ATPase assembly, trafficking, or enzyme activity, we ... [more]

Unknown Dec. 31, 2010; 0(0); [Pubmed: 21196517]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: metal resistance (APO:0000090)

Additional Notes

  • A synthetic genetic array (SGA) screen was used to identify genes that caused an increase in calcium or zinc sensitivity in a vma21QQ voa1::Nat-R double mutant. VOA1 is genetically linked to the VMA21 locus. The vma21QQ mutant has a dysfunctional ER retrieval motif and complete deletion of VOA1 (voa1::Nat-R) results in a decrease in steady-state levels of Vma21p.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SRN2 VMA21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2046BioGRID
2150801

Curated By

  • BioGRID