BAIT

CCC1

L000000234, YLR220W
Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

MRS4

L000001180, YKR052C
Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Genetic and biochemical analysis of high iron toxicity in yeast: Iron toxicity is due to the accumulation of cytosolic iron and occurs under both aerobic and anaerobic conditions.

Lin H, Li L, Jia X, Ward DM, Kaplan J

Iron storage in yeast requires the activity of the vacuolar iron transporter Ccc1. Yeast with an intact CCC1 are resistant to iron toxicity but deletion of CCC1 renders yeast susceptible to iron toxicity. We used genetic and biochemical analysis to identify suppressors of high iron toxicity in ccc1 cells in order to probe the mechanism of high iron toxicity. All ... [more]

J. Biol. Chem. Nov. 29, 2010; 0(0); [Pubmed: 21115478]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: oxidative stress resistance (APO:0000083)
  • phenotype: vegetative growth (APO:0000106)
  • phenotype: metal resistance (APO:0000090)

Additional Notes

  • The experiment involved identifying suppressors of high iron toxicity in ccc1 null mutant cells.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRS4 CCC1
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Low-BioGRID
428906
CCC1 MRS4
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
162867
CCC1 MRS4
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1240518
MRS4 CCC1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
1240496

Curated By

  • BioGRID