Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The tumor suppressor CYLD interacts with TRIP and regulates negatively nuclear factor kappaB activation by tumor necrosis factor.

Regamey A, Hohl D, Liu JW, Roger T, Kogerman P, Toftgard R, Huber M

Cylindromas are benign adnexal skin tumors caused by germline mutations in the CYLD gene. In most cases the second wild-type allele is lost in tumor tissue, suggesting that CYLD functions as tumor suppressor. CYLD is a protein of 956 amino acids harboring a functional deubiquitinating domain at the COOH-terminal end. To shed more light on the function of CYLD, we ... [more]

J. Exp. Med. Dec. 15, 2003; 198(12);1959-64 [Pubmed: 14676304]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CYLD TRAIP	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Regamey A (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CYLD

Gene Ontology Biological Process

Gene Ontology Molecular Function

Lys63-specific deubiquitinase activity [IDA]proline-rich region binding [IPI]protein binding [IPI]protein kinase binding [IPI]structural constituent of ribosome [IBA]ubiquitin-specific protease activity [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

TRAIP