BAIT

LAS17

BEE1, L000003068, L000003581, YOR181W
Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments and a polyproline domain which can nucleate actin filaments independent of Arp2/3; mutants are defective in actin cytoskeleton dependent processes such as: endocytosis, bud site selection and cytokinesis; localizes with the Arp2/3 complex to actin cortical patches; homolog of the Wiskott-Aldrich Syndrome protein (WASP), implicated in severe immunodeficiency
Saccharomyces cerevisiae (S288c)
PREY

CMD1

calmodulin, CaM, L000000365, YBR109C
Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Reconstitution and protein composition analysis of endocytic actin patches.

Michelot A, Costanzo M, Sarkeshik A, Boone C, Yates JR, Drubin DG

Clathrin-actin-mediated endocytosis in yeast involves the progressive assembly of at least 60 different proteins at cortical sites. More than half of these proteins are involved in the assembly of a branched network of actin filaments to provide the forces required for plasma membrane invagination.To gain insights into the regulation of endocytic actin patch dynamics, we developed an in vitro actin ... [more]

Curr. Biol. Nov. 09, 2010; 20(21);1890-9 [Pubmed: 21035341]

Throughput

  • High Throughput

Additional Notes

  • MudPIT and tandem mass spectra analysis were used to identify proteins associated with Las17 functionalized microbeads.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LAS17 CMD1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3642BioGRID
1952702
CMD1 LAS17
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3298BioGRID
1870795
CMD1 LAS17
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High0.1957BioGRID
1870794

Curated By

  • BioGRID