BAIT
SFRP1
2210415K03Rik, AW011917, AW107218, AW742929, sFRP-1
secreted frizzled-related protein 1
GO Process (75)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IGI]
- Wnt signaling pathway, planar cell polarity pathway [IDA]
- actin cytoskeleton organization [ISO]
- anterior/posterior pattern specification [IGI]
- bone trabecula formation [IMP]
- canonical Wnt signaling pathway [ISO]
- cellular response to X-ray [IDA]
- cellular response to estrogen stimulus [IDA, ISO]
- cellular response to fibroblast growth factor stimulus [ISO]
- cellular response to growth factor stimulus [ISO]
- cellular response to heparin [ISO]
- cellular response to tumor necrosis factor [ISO]
- convergent extension involved in somitogenesis [IGI]
- development of primary male sexual characteristics [IGI]
- digestive tract morphogenesis [IGI]
- dorsal/ventral axis specification [ISO]
- female gonad development [IGI]
- hematopoietic progenitor cell differentiation [ISO]
- hematopoietic stem cell differentiation [ISO]
- hemopoiesis [IMP]
- male gonad development [IGI]
- negative regulation of B cell differentiation [IMP, ISO]
- negative regulation of BMP signaling pathway [IGI]
- negative regulation of JUN kinase activity [IGI]
- negative regulation of Wnt signaling pathway [IGI, ISO]
- negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification [ISO]
- negative regulation of androgen receptor signaling pathway [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of bone remodeling [ISO]
- negative regulation of canonical Wnt signaling pathway [IDA, IGI, ISO]
- negative regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell migration [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of epithelial to mesenchymal transition [ISO]
- negative regulation of fibroblast apoptotic process [ISO]
- negative regulation of fibroblast proliferation [ISO]
- negative regulation of gene expression [IMP, ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of ossification [IMP, ISO]
- negative regulation of osteoblast differentiation [IMP]
- negative regulation of osteoblast proliferation [IMP, ISO]
- negative regulation of osteoclast differentiation [IMP, ISO]
- negative regulation of peptidyl-tyrosine phosphorylation [ISO]
- negative regulation of planar cell polarity pathway involved in axis elongation [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- neural crest cell fate commitment [IMP]
- neural tube closure [IGI]
- neural tube development [IGI]
- planar cell polarity pathway involved in neural tube closure [IGI]
- positive regulation of Wnt signaling pathway [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of cell growth [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of epithelial cell proliferation [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of fibroblast apoptotic process [ISO]
- positive regulation of non-canonical Wnt signaling pathway [ISO]
- positive regulation of smoothened signaling pathway [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis [IMP]
- proteolysis [ISO]
- regulation of branching involved in prostate gland morphogenesis [IMP]
- regulation of cell cycle process [ISO]
- regulation of establishment of planar polarity [IGI]
- regulation of ossification [ISO]
- response to drug [IDA, ISO]
- response to organic cyclic compound [ISO]
- somatic stem cell maintenance [IMP]
- somitogenesis [IGI]
- substrate adhesion-dependent cell spreading [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
WNT3A
Wnt-3a, vt, RP23-378H5.2
wingless-type MMTV integration site family, member 3A
GO Process (66)
GO Function (7)
GO Component (2)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- COP9 signalosome assembly [IDA]
- Wnt signaling pathway [IGI]
- Wnt signaling pathway involved in forebrain neuroblast division [IMP]
- anterior/posterior pattern specification [IDA]
- axis elongation involved in somitogenesis [IGI]
- axon guidance [IDA]
- axonogenesis [IDA, IGI]
- canonical Wnt signaling pathway [IDA, IGI, IMP, ISO]
- canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [ISO]
- cardiac muscle cell fate commitment [IGI]
- cell proliferation in forebrain [IDA]
- cell-cell signaling [TAS]
- cellular protein localization [IDA]
- determination of left/right symmetry [IMP]
- dorsal/ventral neural tube patterning [IMP]
- extracellular matrix organization [IDA]
- heart looping [IMP]
- hemopoiesis [IDA]
- hippocampus development [IMP]
- in utero embryonic development [IMP]
- inner ear morphogenesis [IGI]
- mammary gland development [IDA]
- mesoderm development [IMP]
- negative regulation of axon extension involved in axon guidance [IDA]
- negative regulation of fat cell differentiation [IDA, IGI]
- negative regulation of heart induction by canonical Wnt signaling pathway [ISO]
- negative regulation of neurogenesis [IDA]
- negative regulation of neuron projection development [IDA]
- organ morphogenesis [TAS]
- osteoblast differentiation [IGI]
- palate development [ISO]
- paraxial mesodermal cell fate commitment [IMP]
- platelet activation [IDA]
- platelet aggregation [IDA]
- positive regulation of B cell proliferation [IDA]
- positive regulation of canonical Wnt signaling pathway [IDA, ISO]
- positive regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation [IDA]
- positive regulation of cardiac muscle cell differentiation [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of cell proliferation [IDA]
- positive regulation of cell-cell adhesion mediated by cadherin [IDA]
- positive regulation of collateral sprouting in absence of injury [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of cytokine production [IDA]
- positive regulation of dermatome development [ISO]
- positive regulation of mesodermal cell fate specification [ISO]
- positive regulation of neural precursor cell proliferation [IDA]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein binding [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of protein tyrosine kinase activity [IGI]
- positive regulation of receptor activity [ISO]
- positive regulation of receptor internalization [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- post-anal tail morphogenesis [IMP]
- regulation of axonogenesis [IGI]
- regulation of cell differentiation [IDA]
- regulation of microtubule cytoskeleton organization [IGI]
- signal transduction [TAS]
- skeletal muscle cell differentiation [IGI]
- somatic stem cell division [IDA]
- somitogenesis [IMP]
- spinal cord association neuron differentiation [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Wnt3a binds to several sFRPs in the nanomolar range.
Secreted Frizzled-related proteins (sFRPs) are modulators of the Wnt signaling pathway that plays important roles in both embryogenesis and oncogenesis. sFRPs have been proposed to antagonize Wnt activity by binding to Wnts. However, the affinity of this binding is unknown. Here we show, using surface plasmon resonance and purified proteins, that sFRP1, sFRP2, sFRP4, and Frzb bind directly to Wnt3a ... [more]
Biochem. Biophys. Res. Commun. Jun. 15, 2007; 357(4);1119-23 [Pubmed: 17462603]
Throughput
- Low Throughput
Additional Notes
- surface plasmon resonance analysis
Curated By
- BioGRID