BAIT
SFRP2
AI851596, Sdf5
secreted frizzled-related protein 2
GO Process (60)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IGI]
- anterior/posterior pattern specification [IGI]
- apoptotic process [IMP]
- bone morphogenesis [IMP]
- cardiac left ventricle morphogenesis [ISO]
- cartilage development [IMP]
- cellular response to X-ray [IDA]
- cellular response to extracellular stimulus [IDA]
- chondrocyte development [IMP]
- collagen fibril organization [IMP]
- convergent extension involved in axis elongation [IGI]
- development of primary male sexual characteristics [IGI]
- digestive tract morphogenesis [IGI]
- embryonic digit morphogenesis [IMP]
- hematopoietic stem cell proliferation [IDA]
- male gonad development [IGI]
- negative regulation of BMP signaling pathway [IDA, IGI]
- negative regulation of JUN kinase activity [IGI]
- negative regulation of Wnt signaling pathway [IDA, ISO]
- negative regulation of canonical Wnt signaling pathway [IDA, IGI, ISO]
- negative regulation of cardiac muscle cell apoptotic process [IDA]
- negative regulation of cell growth [ISO]
- negative regulation of cell migration [IDA]
- negative regulation of cell proliferation [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of dermatome development [IDA]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of epithelial to mesenchymal transition [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- negative regulation of gene expression [IDA]
- negative regulation of mesodermal cell fate specification [IGI]
- negative regulation of peptidyl-tyrosine phosphorylation [IDA]
- negative regulation of planar cell polarity pathway involved in axis elongation [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IGI]
- neural tube development [IGI]
- outflow tract morphogenesis [ISO]
- patterning of blood vessels [IDA]
- planar cell polarity pathway involved in neural tube closure [IGI]
- positive regulation of angiogenesis [IDA]
- positive regulation of apoptotic process [ISO]
- positive regulation of canonical Wnt signaling pathway [IDA, IGI]
- positive regulation of catenin import into nucleus [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cell proliferation [IDA, ISO]
- positive regulation of endopeptidase activity [IDA]
- positive regulation of fat cell differentiation [ISO]
- positive regulation of osteoblast differentiation [IGI]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of receptor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- post-anal tail morphogenesis [IMP]
- regulation of Wnt signaling pathway [IDA]
- regulation of apoptotic process [IMP]
- regulation of cell proliferation [IMP]
- regulation of establishment of planar polarity [IGI]
- regulation of stem cell division [IDA]
- response to drug [IDA]
- signal transduction [IDA]
- somitogenesis [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
WNT3A
Wnt-3a, vt, RP23-378H5.2
wingless-type MMTV integration site family, member 3A
GO Process (66)
GO Function (7)
GO Component (2)
Gene Ontology Biological Process
- BMP signaling pathway [ISO]
- COP9 signalosome assembly [IDA]
- Wnt signaling pathway [IGI]
- Wnt signaling pathway involved in forebrain neuroblast division [IMP]
- anterior/posterior pattern specification [IDA]
- axis elongation involved in somitogenesis [IGI]
- axon guidance [IDA]
- axonogenesis [IDA, IGI]
- canonical Wnt signaling pathway [IDA, IGI, IMP, ISO]
- canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [ISO]
- cardiac muscle cell fate commitment [IGI]
- cell proliferation in forebrain [IDA]
- cell-cell signaling [TAS]
- cellular protein localization [IDA]
- determination of left/right symmetry [IMP]
- dorsal/ventral neural tube patterning [IMP]
- extracellular matrix organization [IDA]
- heart looping [IMP]
- hemopoiesis [IDA]
- hippocampus development [IMP]
- in utero embryonic development [IMP]
- inner ear morphogenesis [IGI]
- mammary gland development [IDA]
- mesoderm development [IMP]
- negative regulation of axon extension involved in axon guidance [IDA]
- negative regulation of fat cell differentiation [IDA, IGI]
- negative regulation of heart induction by canonical Wnt signaling pathway [ISO]
- negative regulation of neurogenesis [IDA]
- negative regulation of neuron projection development [IDA]
- organ morphogenesis [TAS]
- osteoblast differentiation [IGI]
- palate development [ISO]
- paraxial mesodermal cell fate commitment [IMP]
- platelet activation [IDA]
- platelet aggregation [IDA]
- positive regulation of B cell proliferation [IDA]
- positive regulation of canonical Wnt signaling pathway [IDA, ISO]
- positive regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation [IDA]
- positive regulation of cardiac muscle cell differentiation [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of cell proliferation [IDA]
- positive regulation of cell-cell adhesion mediated by cadherin [IDA]
- positive regulation of collateral sprouting in absence of injury [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of cytokine production [IDA]
- positive regulation of dermatome development [ISO]
- positive regulation of mesodermal cell fate specification [ISO]
- positive regulation of neural precursor cell proliferation [IDA]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein binding [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of protein tyrosine kinase activity [IGI]
- positive regulation of receptor activity [ISO]
- positive regulation of receptor internalization [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- post-anal tail morphogenesis [IMP]
- regulation of axonogenesis [IGI]
- regulation of cell differentiation [IDA]
- regulation of microtubule cytoskeleton organization [IGI]
- signal transduction [TAS]
- skeletal muscle cell differentiation [IGI]
- somatic stem cell division [IDA]
- somitogenesis [IMP]
- spinal cord association neuron differentiation [IDA, IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Wnt3a binds to several sFRPs in the nanomolar range.
Secreted Frizzled-related proteins (sFRPs) are modulators of the Wnt signaling pathway that plays important roles in both embryogenesis and oncogenesis. sFRPs have been proposed to antagonize Wnt activity by binding to Wnts. However, the affinity of this binding is unknown. Here we show, using surface plasmon resonance and purified proteins, that sFRP1, sFRP2, sFRP4, and Frzb bind directly to Wnt3a ... [more]
Biochem. Biophys. Res. Commun. Jun. 15, 2007; 357(4);1119-23 [Pubmed: 17462603]
Throughput
- Low Throughput
Additional Notes
- surface plasmon resonance analysis
Curated By
- BioGRID