BAIT

CTNNB2

wu:fa12h09, wu:fc16f05, zgc:65770
catenin, beta 2
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Danio rerio
PREY

TOB1A

tob1
transducer of ERBB2, 1a
GO Process (4)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Danio rerio

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity.

Xiong B, Rui Y, Zhang M, Shi K, Jia S, Tian T, Yin K, Huang H, Lin S, Zhao X, Chen Y, Chen YG, Lin SC, Meng A

Maternal beta-catenin and Nodal signals are essential for the formation of the dorsal organizer, which, in turn, induces neural and other dorsal tissue development in vertebrate embryos. Tob (Transducer of ErbB2) proteins possess antiproliferative properties and are known to influence BMP signaling, but their relationship to other signaling pathways and to embryonic patterning in general was unclear. In this study, ... [more]

Dev. Cell Aug. 01, 2006; 11(2);225-38 [Pubmed: 16890162]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: embryonic structure (ZFA:0001105) [dorsalized (PATO:0000620)]

Additional Notes

  • Overexpression of beta-catenin-delta-N terminal in Zebrafish embryos leads to dorsalized phenotypes. Co-injection with tob1a mRNA suppresses the dorsalized phenotype.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOB1A CTNNB2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CTNNB2 TOB1A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CTNNB2 TOB1A
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
503353
TOB1A CTNNB2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID