BAIT

TSSK6

CT72, SSTK, TSSK4, FKSG82
testis-specific serine kinase 6
GO Process (2)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HSPA4

APG-2, HEL-S-5a, HS24/P52, HSPH2, RY, hsp70, hsp70RY
heat shock 70kDa protein 4
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility.

Spiridonov NA, Wong L, Zerfas PM, Starost MF, Pack SD, Paweletz CP, Johnson GR

Here we describe and characterize a small serine/threonine kinase (SSTK) which consists solely of the N- and C-lobes of a protein kinase catalytic domain. SSTK protein is highly conserved among mammals, and no close homologues were found in the genomes of nonmammalian organisms. SSTK specifically interacts with HSP90-1beta, HSC70, and HSP70 proteins, and this association appears to be required for ... [more]

Mol. Cell. Biol. May. 01, 2005; 25(10);4250-61 [Pubmed: 15870294]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181)

Additional Notes

  • hs; exogenous expression of bait

Curated By

  • BioGRID