BAIT

CDC42

CDC42Hs, G25K, RP1-224A6.5
cell division cycle 42
GO Process (25)
GO Function (7)
GO Component (14)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Activated Cdc42 sequesters c-Cbl and prevents EGF receptor degradation.

Wu WJ, Tu S, Cerione RA

Cdc42 is a Ras-related protein that has been implicated in the control of normal cell growth, and when improperly regulated, in cellular transformation and invasiveness. A variety of extracellular stimuli, including epidermal growth factor (EGF), activate Cdc42. Here, we show that activation of Cdc42 protects the EGF receptor from the negative regulatory activity of the c-Cbl ubiquitin ligase. Activated Cdc42 ... [more]

Cell Sep. 19, 2003; 114(6);715-25 [Pubmed: 14505571]

Throughput

  • Low Throughput

Curated By

  • BioGRID