Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Androgen-receptor coregulators mediate the suppressive effect of androgen signals on vitamin D receptor activity.

Ting HJ, Bao BY, Hsu CL, Lee YF

Overexpression of androgen receptors (AR) in PC-3 cell, and treatment of 5alpha-dihydrotestosterone in LNCaP cells lead to the suppression of VDR transactivation. Competition for shared coregulators between AR and VDR is one possible mechanism to explain the suppressive effect of androgen-AR signals on VDR activity. Among the AR coregulators we tested, ARA54, ARA70, supervillin, and gelsolin were found to enhance ... [more]

Endocrine Feb. 01, 2005; 26(1);1-9 [Pubmed: 15805579]

Throughput

  • Low Throughput

Curated By

  • BioGRID