PURA
Gene Ontology Biological Process
- DNA unwinding involved in DNA replication [ISO]
- apoptotic process [NAS]
- cell differentiation [NAS]
- cell proliferation [NAS]
- mitotic cell cycle checkpoint [NAS]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of translation [IDA]
- nervous system development [IMP]
- positive regulation of cell proliferation [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- SMAD binding [IDA]
- double-stranded DNA binding [IDA]
- double-stranded telomeric DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- purine-rich negative regulatory element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- single-stranded DNA binding [IDA]
- transcription factor binding [IPI]
- translation repressor activity, nucleic acid binding [IDA]
- DNA binding [IDA]
- SMAD binding [IDA]
- double-stranded DNA binding [IDA]
- double-stranded telomeric DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- purine-rich negative regulatory element binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- single-stranded DNA binding [IDA]
- transcription factor binding [IPI]
- translation repressor activity, nucleic acid binding [IDA]
CCND1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA, ISO]
- Leydig cell differentiation [ISO]
- Wnt signaling pathway [IDA]
- canonical Wnt signaling pathway [IMP]
- cellular response to DNA damage stimulus [ISO]
- cellular response to organic substance [IDA]
- endoplasmic reticulum unfolded protein response [IDA]
- fat cell differentiation [IDA]
- lactation [IGI, IMP]
- mammary gland alveolus development [IGI, IMP]
- mammary gland epithelial cell proliferation [IGI]
- mitotic G1 DNA damage checkpoint [ISO]
- negative regulation of Wnt signaling pathway [IMP]
- negative regulation of cell cycle arrest [ISO]
- negative regulation of epithelial cell differentiation [IGI]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of G2/M transition of mitotic cell cycle [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity [ISO]
- positive regulation of mammary gland epithelial cell proliferation [IGI]
- positive regulation of protein phosphorylation [ISO]
- protein phosphorylation [IDA]
- re-entry into mitotic cell cycle [IDA]
- regulation of G1/S transition of mitotic cell cycle [IGI]
- regulation of cell cycle [IDA]
- regulation of protein kinase activity [IDA]
- response to UV-A [ISO]
- response to organonitrogen compound [ISO]
Gene Ontology Molecular Function- cyclin-dependent protein serine/threonine kinase regulator activity [IDA]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- kinase activity [IDA]
- proline-rich region binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [IPI, ISO]
- transcription corepressor activity [ISO]
- transcription factor binding [ISO]
- cyclin-dependent protein serine/threonine kinase regulator activity [IDA]
- enzyme binding [ISO]
- histone deacetylase binding [ISO]
- kinase activity [IDA]
- proline-rich region binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [IPI, ISO]
- transcription corepressor activity [ISO]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Functional interaction of Puralpha with the Cdk2 moiety of cyclin A/Cdk2.
Puralpha is a sequence-specific single-stranded nucleic acid-binding protein and a member of the highly conserved Pur family. Puralpha has been shown to colocalize with cyclin A/Cdk2 and to coimmunoprecipitate with cyclin A during S-phase. Here we show that this interaction is mediated by a specific affinity of Puralpha for Cdk2. In pull-down assays GST-Puralpha efficiently binds Cdk2 and Cdk1, binds ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID