BAIT

DPB4

S000007463, YDR121W
Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
Saccharomyces cerevisiae (S288c)
PREY

HDA2

PLO2, YDR295C
Subunit of the HDA1 histone deacetylase complex; possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; relocalizes to the cytosol in response to hypoxia
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Genetic Analysis Implicates the Set3/Hos2 Histone Deacetylase in the Deposition and Remodeling of Nucleosomes Containing H2A.Z.

Hang M, Smith MM

Histone variants and histone modification complexes act to regulate the functions of chromatin. In Saccharomyces cerevisiae the histone variant H2A.Z is encoded by HTZ1. Htz1 is dispensable for viability in budding yeast, but htz1Δ is synthetic sick or lethal with the null alleles of about 200 non-essential genes. One of the strongest of these interactions is with the deletion of ... [more]

Unknown Feb. 04, 2011; 0(0); [Pubmed: 21288874]

Throughput

  • High Throughput

Ontology Terms

  • colony size (APO:0000063)

Additional Notes

  • High Throughput: A modified Synthetic Genetic Array (SGA) analysis involving triple mutants was carried out to assess the genome-wide suppression of htz1 negative genetic interactions. A double mutant query strain carrying a deletion of hda2 (suppressor) and an htz1 deletion was crossed against mini-arrays containing strains with known gene deletions that have negative genetic interactions with htz1 null mutants.

Curated By

  • BioGRID