BAIT

NFKB2

CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p52
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Homo sapiens
PREY

PSMD11

Rpn6, S9, p44.5
proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
GO Process (24)
GO Function (1)
GO Component (7)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

S9, a 19 S proteasome subunit interacting with ubiquitinated NF-kappaB2/p100.

Fong A, Zhang M, Neely J, Sun SC

Proteasome-mediated processing of the nfkappab2 gene product p100 is a regulated event that generates the NF-kappaB subunit p52. This event can be induced through p100 phosphorylation by a signaling pathway involving the nuclear factor-kappaB-inducing kinase (NIK). The C-terminal region of p100, which contains its phosphorylation site and a death domain, plays a pivotal role in regulating the processing of p100. ... [more]

J. Biol. Chem. Oct. 25, 2002; 277(43);40697-702 [Pubmed: 12185077]

Throughput

  • Low Throughput

Curated By

  • BioGRID