ETS2
Gene Ontology Biological Process
- cell differentiation [IBA]
- mesoderm development [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- primitive streak formation [IMP]
- regulation of transcription from RNA polymerase II promoter [IBA]
- transcription from RNA polymerase II promoter [IBA]
Gene Ontology Molecular Function- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- glucocorticoid receptor binding [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [ISO]
- DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- glucocorticoid receptor binding [ISO]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
- sequence-specific DNA binding transcription factor activity [ISO]
CREBBP
Gene Ontology Biological Process
- N-terminal peptidyl-lysine acetylation [ISO]
- cell proliferation [ISO]
- germ-line stem cell maintenance [IMP]
- histone acetylation [IDA, ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
- DNA binding [IDA]
- MRF binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- SMAD binding [ISO]
- acetyltransferase activity [IDA, ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA]
- core promoter proximal region sequence-specific DNA binding [ISO]
- histone acetyltransferase activity [IDA, ISO]
- p53 binding [ISO]
- peroxisome proliferator activated receptor binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [ISO]
- transcription coactivator activity [ISO, TAS]
- transcription factor binding [IPI, ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ras/mitogen-activated protein kinase signaling activates Ets-1 and Ets-2 by CBP/p300 recruitment.
Cell signaling affects gene expression by regulating the activity of transcription factors. Here, we report that mitogen-activated protein kinase (MAPK) phosphorylation of Ets-1 and Ets-2, at a conserved site N terminal to their Pointed (PNT) domains, resulted in enhanced transactivation by preferential recruitment of the coactivators CREB binding protein (CBP) and p300. We discovered this phosphorylation-augmented interaction in an unbiased ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CREBBP ETS2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID