BAIT
IRS1
G972R, IRS-1
insulin receptor substrate 1
GO Process (28)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- cellular response to insulin stimulus [IDA, ISO]
- insulin receptor signaling pathway [IBA, ISO]
- insulin secretion [IMP]
- insulin-like growth factor receptor signaling pathway [ISO]
- lipid catabolic process [IMP]
- mammary gland development [IGI]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of somatostatin secretion [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [IGI]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [ISO]
- positive regulation of mesenchymal cell proliferation [IGI]
- positive regulation of phosphatidylinositol 3-kinase activity [IMP, ISO]
- positive regulation of phosphorylation [ISO]
- protein heterooligomerization [ISO]
- protein kinase B signaling [IGI]
- protein localization to nucleus [IDA, IMP]
- regulation of gene expression [IMP]
- response to caffeine [ISO]
- response to insulin [ISO]
- response to peptide hormone [ISO]
Gene Ontology Molecular Function- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
HDAC2
D10Wsu179e, YAF1, Yy1bp, mRPD3
histone deacetylase 2
GO Process (43)
GO Function (18)
GO Component (13)
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [ISO]
- cardiac muscle cell development [IMP]
- cellular response to hydrogen peroxide [ISO]
- chromatin modification [TAS]
- circadian regulation of gene expression [IDA]
- dendrite development [IMP]
- embryonic digit morphogenesis [IGI]
- epidermal cell differentiation [IGI]
- eyelid development in camera-type eye [IGI]
- fungiform papilla formation [IGI]
- hair follicle placode formation [IGI]
- hippocampus development [IGI]
- histone H3 deacetylation [IDA, ISO]
- histone H4 deacetylation [IDA]
- histone deacetylation [IGI, ISO, TAS]
- maintenance of chromatin silencing [ISO]
- negative regulation of DNA binding [ISO]
- negative regulation of apoptotic process [IGI]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cardiac muscle cell proliferation [IMP]
- negative regulation of dendritic spine development [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [IGI]
- negative regulation of neuron projection development [IDA, ISO]
- negative regulation of peptidyl-lysine acetylation [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IGI, ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- neuron differentiation [IGI]
- odontogenesis of dentin-containing tooth [IGI]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of interleukin-1 production [ISO]
- positive regulation of oligodendrocyte differentiation [IGI, ISO]
- positive regulation of proteolysis [ISO]
- positive regulation of receptor biosynthetic process [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- positive regulation of tyrosine phosphorylation of Stat3 protein [ISO]
- protein deacetylation [IDA]
- regulation of protein deacetylation [IGI]
- regulation of protein kinase B signaling [IMP]
- regulation of sarcomere organization [IMP]
Gene Ontology Molecular Function- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [ISO]
- deacetylase activity [IDA]
- enzyme binding [IPI, ISO]
- heat shock protein binding [ISO]
- histone deacetylase activity [IDA, ISO, TAS]
- nucleosomal DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein deacetylase activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO, TAS]
- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [ISO]
- deacetylase activity [IDA]
- enzyme binding [IPI, ISO]
- heat shock protein binding [ISO]
- histone deacetylase activity [IDA, ISO, TAS]
- nucleosomal DNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein deacetylase activity [IDA, ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [ISO, TAS]
Gene Ontology Cellular Component
- ESC/E(Z) complex [ISO]
- NuRD complex [ISO]
- Sin3 complex [ISO]
- chromatin [ISO]
- cytoplasm [ISO, TAS]
- heterochromatin [IDA]
- histone deacetylase complex [TAS]
- nuclear chromatin [IDA, ISO]
- nucleus [IDA, ISO]
- protein complex [ISO]
- replication fork [IDA]
- transcription factor complex [IPI]
- transcriptional repressor complex [IPI]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation.
BACKGROUND: Insulin receptor substrate (IRS) proteins are key moderators of insulin action. Their specific regulation determines downstream protein-protein interactions and confers specificity on growth factor signalling. Regulatory mechanisms that have been identified include phosphorylation of IRS proteins on tyrosine and serine residues and ubiquitination of lysine residues. This study investigated other potential molecular mechanisms of IRS-1 regulation. RESULTS: Using the ... [more]
BMC Biol. Nov. 04, 2004; 2(0);23 [Pubmed: 15522123]
Throughput
- Low Throughput
Curated By
- BioGRID