ASF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CAF1
Gene Ontology Biological Process
- DNA repair [TAS]
- DNA replication-dependent nucleosome assembly [NAS]
- chromatin assembly [IDA, ISS]
- chromatin silencing [IPI]
- dendrite morphogenesis [IMP]
- eggshell chorion gene amplification [IC]
- histone acetylation [IDA, ISS]
- histone methylation [IDA]
- mitotic cytokinesis [IMP]
- muscle organ development [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- neuron development [IMP]
- neuron projection morphogenesis [IMP]
- nucleosome assembly [IDA, NAS]
- nucleosome mobilization [IDA]
- nucleosome positioning [IDA]
- positive regulation of cell proliferation [IMP]
- regulation of histone H3-K27 methylation [IMP]
- regulation of mitotic cell cycle [IMP]
- segment specification [IMP]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors.
The assembly of newly synthesized DNA into chromatin is essential for normal growth, development, and differentiation. To gain a better understanding of the assembly of chromatin during DNA synthesis, we identified, cloned, and characterized the 180- and 105-kDa polypeptides of Drosophila chromatin assembly factor 1 (dCAF-1). The purified recombinant p180+p105+p55 dCAF-1 complex is active for DNA replication-coupled chromatin assembly. Furthermore, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ASF1 CAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CAF1 ASF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CAF1 ASF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CAF1 ASF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID