BAIT

COG1

COD3, LDB11, SEC36, YGL223C
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
GO Process (4)
GO Function (1)
GO Component (1)
Saccharomyces cerevisiae (S288c)
PREY

ATG20

CVT20, SNX42, YDL113C
Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
GO Process (4)
GO Function (1)
GO Component (3)
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.

Yen WL, Shintani T, Nair U, Cao Y, Richardson BC, Li Z, Hughson FM, Baba M, Klionsky DJ

Macroautophagy is a catabolic pathway used for the turnover of long-lived proteins and organelles in eukaryotic cells. The morphological hallmark of this process is the formation of double-membrane autophagosomes that sequester cytoplasm. Autophagosome formation is the most complex part of macroautophagy, and it is a dynamic event that likely involves vesicle fusion to expand the initial sequestering membrane, the phagophore; ... [more]

J. Cell Biol. Jan. 11, 2010; 188(1);101-14 [Pubmed: 20065092]

Throughput

  • Low Throughput

Curated By

  • BioGRID