BAIT

HOS3

L000004093, YPL116W
Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
Saccharomyces cerevisiae (S288c)
PREY

MSL1

YIB9, YIB9w, L000004502, YIR009W
U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangements.

Gunderson FQ, Merkhofer EC, Johnson TL

Assembly of the spliceosome onto pre-mRNA is a dynamic process involving the ordered exchange of snRNPs to form the catalytically active spliceosome. These ordered rearrangements have recently been shown to occur cotranscriptionally, while the RNA polymerase is still actively engaged with the chromatin template. We previously demonstrated that the histone acetyltransferase Gcn5 is required for U2 snRNP association with the ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 01, 2011; 108(5);2004-9 [Pubmed: 21245291]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • deletion of msl1 increases the temperature sensitivity of a hos2/hos3 mutant
  • genetic complex

Curated By

  • BioGRID