BAIT

MRGBP

C20orf20, Eaf7, MRG15BP, URCC4
MRG/MORF4L binding protein
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

DIP2A

C21orf106, DIP2
DIP2 disco-interacting protein 2 homolog A (Drosophila)
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Probabilistic assembly of human protein interaction networks from label-free quantitative proteomics.

Sardiu ME, Cai Y, Jin J, Swanson SK, Conaway RC, Conaway JW, Florens L, Washburn MP

Large-scale affinity purification and mass spectrometry studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes. However, the development of such networks for human proteins has been slowed by the high cost and significant technical challenges associated with systematic studies of protein interactions. To address this challenge, we have developed a method ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 05, 2008; 105(5);1454-9 [Pubmed: 18218781]

Throughput

  • Low Throughput

Curated By

  • BioGRID