BAIT

CDK5R1

Cdk5r, D11Bwg0379e, p25, p35, RP23-252E4.5
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
GO Process (30)
GO Function (10)
GO Component (15)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cdk5/p35 phosphorylates mSds3 and regulates mSds3-mediated repression of transcription.

Li Z, David G, Hung KW, DePinho RA, Fu AK, Ip NY

Cyclin-dependent kinase 5 (Cdk5), a serine/threonine kinase that displays kinase activity predominantly in neurons, is activated by two non-cyclin activators, p35 or p39. Here, we report a physical and functional interaction between the Cdk5/p35 complex and mouse Sds3 (mSds3), an essential component of mSin3-histone deacetylase (HDAC) co-repressor complex. mSds3 binds to p35 both in vitro and in vivo, enabling active ... [more]

J. Biol. Chem. Dec. 24, 2004; 279(52);54438-44 [Pubmed: 15489224]

Throughput

  • Low Throughput

Curated By

  • BioGRID